Phospholipase D antibody (Biotin)

Catalog No. ABIN459096

Product Details anti-Phospholipase D Antibody (Biotin)

Target: Phospholipase D (PLD)

Reactivity: Streptomyces chromofuscus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase D antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to phospholipase D from streptomyces chromofuscus.

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Phospholipase D isolated and purified from streptomyces chromofuscus. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immuno-fluorescence or histochemical techniques).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 4.7. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at am bient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for Phospholipase D

Target: Phospholipase D (PLD)

Alternative Name: Phospholipase D (PLD Products)

Synonyms: CG12110 antibody, Dmel\\CG12110 antibody, PLD antibody, dPLD antibody, dPld antibody, pld antibody, pld1 antibody, PHOSPHOLIPASE D antibody, PHOSPHOLIPASE D P1 antibody, PHOSPHOLIPASE D ZETA 1 antibody, PHOSPHOLIPASE D ZETA1 antibody, PLD ZETA 1 antibody, PLDZ1 antibody, PLDZETA1 antibody, phospholipase D P1 antibody, Phospholipase D antibody, phospholipase D P1 antibody, phospholipase antibody, phospholipase D antibody, phospholipase d antibody, glycosylphosphatidylinositol specific phospholipase D1 antibody, Pld antibody, PLDP1 antibody, RR_RS10410 antibody, CNE01480 antibody, pld antibody, plD antibody, GPLD1 antibody

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Pathways: Response to Water Deprivation, ER-Nucleus Signaling