Neutral Protease antibody (Biotin)
Reactivity: Bacillus polymyxa
IF, WB, ELISA
Host: Rabbit
Polyclonal
Biotin
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- Target See all Neutral Protease products
- Neutral Protease
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Reactivity
- Bacillus polymyxa
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Neutral Protease antibody is conjugated to Biotin
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Application
- Immunofluorescence (IF), Western Blotting (WB), ELISA
- Specificity
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- Characteristics
- Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to neutral protease from bacillus polymyxa.
- Purification
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- Immunogen
- Neutral protease isolated and purified from Bacillus polymyxa. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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- Application Notes
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immuno-fluorescence or histochemical techniques).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 5.1. No foreign proteins added.
- Buffer
- Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Pri or to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Target
- Neutral Protease
- Abstract
- Neutral Protease Products
- Synonyms
- BA0599 antibody, bacillolysin antibody, Neutral protease antibody, neutral protease antibody, BA_0599 antibody, VV2_0974 antibody, BCE_0667 antibody, BAS0567 antibody, npr antibody
- Background
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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