Isocitrate Dehydrogenase antibody (Biotin)

Catalog No. ABIN459052

Product Details anti-Isocitrate Dehydrogenase Antibody (Biotin)

Target: Isocitrate Dehydrogenase (IDH)

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Isocitrate Dehydrogenase antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to isocitrate dehydrogenase from porcine heart

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Isocitrate dehydrogenase isolated and purified from porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 6.5. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for Isocitrate Dehydrogenase

Target: Isocitrate Dehydrogenase (IDH)

Alternative Name: Iso Citrate Dehydrogenase (IDH Products)

Synonyms: CG7176 antibody, CT22171 antibody, Dmel\\CG7176 antibody, IDH antibody, IDH-NADP antibody, Idh-NADP antibody, idh antibody, isocitrate dehydrogenase antibody, l(3)L3852 antibody, F20D21.16 antibody, F20D21_16 antibody, SMU.672 antibody, icd antibody, Isocitrate dehydrogenase antibody, isocitrate dehydrogenase antibody, isocitrate dehydrogenase, NADP-dependent antibody, Monomeric isocitrate dehydrogenase antibody, ankyrin repeat domain 26-like antibody, Idh antibody, ICDH antibody, idh antibody, icd antibody, SSO_RS10660 antibody, JK_RS08640 antibody, LOC618625 antibody

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.