GUK1 antibody (Biotin)

Catalog No. ABIN459048

Product Details anti-GUK1 Antibody (Biotin)

Target: GUK1 (Guanylate Kinase 1 (GUK1))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GUK1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to guanylate kinase from porcine brain

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Guanylate kinase isolated and purified from porcine brain. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 6.6. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for GUK1

Target: GUK1 (Guanylate Kinase 1 (GUK1))

Alternative Name: Guanylate Kinase (GUK1 Products)

Synonyms: GMK antibody, AA409738 antibody, AL033299 antibody, AL033300 antibody, AV026611 antibody, Gmk antibody, Guk-1 antibody, guk1 antibody, zgc:73128 antibody, zgc:73217 antibody, gmk antibody, GUK1 antibody, AGK1 antibody, T11A7.23 antibody, guanylate kinase 1 antibody, DDBDRAFT_0215291 antibody, DDBDRAFT_0230100 antibody, DDB_0215291 antibody, DDB_0230100 antibody, sb:cb295 antibody, zgc:86776 antibody, guanylate kinase 1 antibody, guanylate kinase (GUK1) antibody, guanylate kinase antibody, guanylate kinase 1b antibody, guanylate kinase 1 L homeolog antibody, guanylate kinase 1a antibody, GUK1 antibody, Guk1 antibody, guk1b antibody, guk1 antibody, guk1.L antibody, GK-1 antibody, gmk antibody, gmkA antibody, Mrub_2093 antibody, Arnit_0783 antibody, Ndas_3097 antibody, Mesil_1319 antibody, Slip_0854 antibody, Olsu_0986 antibody, Acear_1451 antibody, AOR_1_2886174 antibody, guk1a antibody

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Pathways: Nucleotide Phosphorylation, ER-Nucleus Signaling, Ribonucleoside Biosynthetic Process