Endonuclease antibody (Biotin)
Reactivity: Neurospora crassa
IF, WB, ELISA
Host: Rabbit
Polyclonal
Biotin
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- Target See all Endonuclease products
- Endonuclease
- Reactivity
- Neurospora crassa
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Endonuclease antibody is conjugated to Biotin
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Application
- Immunofluorescence (IF), Western Blotting (WB), ELISA
- Specificity
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- Characteristics
- Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to endonuclease from Neurospora crassa.
- Purification
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- Immunogen
- Endonuclease isolated and purified from Neurospora crassa. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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- Application Notes
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immuno-fluorescence or histochemical techniques).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 5.0. No foreign proteins added.
- Buffer
- Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Pri or to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Target
- Endonuclease
- Abstract
- Endonuclease Products
- Background
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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