Collagenase antibody (Biotin)
Reactivity: Clostridium histolyticum
ELISA, IF, WB
Host: Rabbit
Polyclonal
Biotin
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- Target See all Collagenase products
- Collagenase
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Reactivity
- Clostridium histolyticum
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Collagenase antibody is conjugated to Biotin
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Application
- ELISA, Immunofluorescence (IF), Western Blotting (WB)
- Specificity
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- Characteristics
- Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to collagenase from Clostridium histolyticum
- Purification
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- Immunogen
- Collagenase isolated and purified from Clostridium histolyticum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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- Application Notes
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 7.4. No foreign proteins added.
- Buffer
- Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Target
- Collagenase
- Abstract
- Collagenase Products
- Synonyms
- collagenase antibody, RB3913 antibody, BPSS0666 antibody, Sbal_3732 antibody, Bcer98_0486 antibody, Shew185_0630 antibody, Shal_3056 antibody, Sbal195_0657 antibody, Lbys_3550 antibody, Palpr_2084 antibody
- Background
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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