This Cholesterol Esterase antibody is conjugated to Biotin
Application
Western Blotting (WB), ELISA, Immunofluorescence (IF)
Specificity
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Characteristics
Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to cholesterol esterase from Candida cylindracea
Purification
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
Immunogen
Cholesterol esterase isolated and purified from Candida cylindracea. Freund’s complete adjuvant is used in the first step of the immunization procedure.
CEL
Reactivity: Human
Host: Rabbit
Polyclonal
FITC
Application Notes
This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
Restrictions
For Research Use only
Format
Lyophilized
Concentration
IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 6.5. No foreign proteins added.
Buffer
Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
SC8F4.24 antibody, BAL antibody, BSDL antibody, BSSL antibody, CELL antibody, CEase antibody, FAP antibody, FAPP antibody, LIPA antibody, MODY8 antibody, 1810036E18Rik antibody, Bal antibody, Bssl antibody, carboxyl ester lipase antibody, cholesterol esterase antibody, hypothetical protein antibody, CEL antibody, SCO5420 antibody, Tfu_2331 antibody, SARE_RS01070 antibody, RSal33209_1252 antibody, Caci_5976 antibody, Ndas_0054 antibody, Micau_5972 antibody, ML5_2523 antibody, Cel antibody, cel antibody
Background
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.