PFKM antibody

Catalog No. ABIN458975

Product Details anti-PFKM Antibody

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Reactivity: Rabbit

Host: Sheep

Clonality: Polyclonal

Conjugate: This PFKM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Affinity purified antibodies of polyclonal sheep antiserum to fructose-6-phosphate kinase from rabbit muscle

Purification: Specific polyclonal antibody fractions were prepared by solid phase affinity chromatography.

Immunogen: Fructose-6-phosphate kinase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: PAb concentration 1 mg/0.5ml. No foreign proteins added.

Buffer: Purified hyperimmune sheep IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Alternative Name: Fructose-6-phosphate Kinase (PFKM Products)

Synonyms: GSD7 antibody, PFK-1 antibody, PFK1 antibody, PFKA antibody, PFKX antibody, AI131669 antibody, PFK-A antibody, PFK-M antibody, Pfk-4 antibody, Pfk4 antibody, Pfka antibody, Pfkx antibody, Pfk-M antibody, M-PFK antibody, PFKM antibody, pfkm antibody, zgc:92344 antibody, pfk antibody, im:7137110 antibody, wu:fc58g11 antibody, phosphofructokinase, muscle antibody, phosphofructokinase, platelet antibody, phosphofructokinase, muscle a antibody, phosphofructokinase, muscle S homeolog antibody, phosphofructokinase, muscle b antibody, PFKM antibody, Pfkm antibody, PFKP antibody, pfkma antibody, pfkm.S antibody, pfkmb antibody

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect