HRP antibody

Catalog No. ABIN458842

Product Details anti-HRP Antibody

Target: HRP (Horseradish Peroxidase (HRP))

Reactivity: Horseradish

Host: Mouse

Clonality: Monoclonal

Conjugate: This HRP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This antiserum has not been tested for cross-reactivity.

Characteristics: Purified monoclonal mouse antibody to horseradish peroxidase

Purification: Purified

Immunogen: Highly purified horseradish peroxidase

Clone: NI 91

Isotype: IgG1 kappa

Application Details

Application Notes: TAs a control in the tests where mouse IgG1 (with irrelevant specificity) should be used to show the presence of that given mouse isotype. In indirect tests as reagent to horseradish peroxidase or to potentiatethe visualisation of a peroxidase labelled conjugate in the appropriate material. As secondary reagent a labelled anti mouse IgG will serve for the detection. This system can be further be potentiated by the use of anti-FITC or anti-TRITC conjugates. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG concentration is 0.4 mg/ml. No foreign proteins added.

Buffer: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 20 °C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.

Target Details for HRP

Target: HRP (Horseradish Peroxidase (HRP))

Alternative Name: Horseradish Peroxidase (HRP Products)

Background: The reactivity of the antiserum is directed to horseradish as tested in direct binding enzyme immunoassay, ELISA and immunoblotting.