Glucuronidase beta antibody

Catalog No. ABIN458831

Product Details anti-Glucuronidase beta Antibody

Target: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glucuronidase beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: IgG fraction of polyclonal rabbit antiserum to β-glucuronidase from Escherichia coli

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: beta -Glucuronidase is isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. No foreign proteins added.

Buffer: Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

References for anti-Glucuronidase beta antibody (ABIN458831)

Mickoleit, Rosenfeldt, Toro-Nahuelpan, Schaffer, Schenk, Plitzko, Schüler: "High-Yield Production, Characterization, and Functionalization of Recombinant Magnetosomes in the Synthetic Bacterium Rhodospirillum rubrum "magneticum"." in: Advanced biology, pp. e2101017, (2021) (PubMed).

Mickoleit, Lanzloth, Schüler: "A Versatile Toolkit for Controllable and Highly Selective Multifunctionalization of Bacterial Magnetic Nanoparticles." in: Small (Weinheim an der Bergstrasse, Germany), pp. e1906922, (2020) (PubMed).

Target Details for Glucuronidase beta

Target: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Alternative Name: beta-glucuronidase (GUSB Products)

Synonyms: GUSB antibody, beta-GUS antibody, gus antibody, BG antibody, MPS7 antibody, AI747421 antibody, Gur antibody, Gus antibody, Gus-r antibody, Gus-s antibody, Gus-t antibody, Gus-u antibody, Gut antibody, asd antibody, g antibody, Ac2-223 antibody, si:ch211-160e1.7 antibody, si:ct573103.7 antibody, glucuronidase beta antibody, beta-D-glucuronidase antibody, beta-glucuronidase antibody, beta glucuronidase antibody, glucuronidase, beta antibody, GUSB antibody, bglR antibody, SSO_RS14735 antibody, Glu antibody, Gusb antibody, gusb antibody

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Pathways: Glycosaminoglycan Metabolic Process