alpha-L-Fucosidase (Fuca) antibody (Biotin)

Catalog No. ABIN458812

Product Details

Target: alpha-L-Fucosidase (Fuca)

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: Immunofluorescence (IF), ELISA, Western Blotting (WB)

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to α-L-fucosidase from bovine kidney

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: alpha -L-Fucosidase isolated and purified from bovine kidney. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.3. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details

Target: alpha-L-Fucosidase (Fuca)

Alternative Name: alpha-L-fucosidase (Fuca Products)

Synonyms: FUCA antibody, CG33047 antibody, CG6128 antibody, DmFuca antibody, Dmel\\CG6128 antibody, Fuca_Dm antibody, alpha-L-fucosidase 1 antibody, alpha-L-fucosidase antibody, Alpha-L-fucosidase antibody, FUCA1 antibody, Fuca antibody, Oter_2972 antibody, Athe_2072 antibody, Kfla_2590 antibody, ZPR_4363 antibody, AMED_3093 antibody, TM0306 antibody, fucA1 antibody, CTN_0382 antibody, Coch_2160 antibody, COB47_1843 antibody

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.