AGLU antibody (Biotin)

Catalog No. ABIN458811

Product Details anti-AGLU Antibody (Biotin)

Target: AGLU (alpha-Glucosidase (AGLU))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGLU antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to α-glucosidase from baker’s yeast

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: alpha -Glucosidase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 6.2. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for AGLU

Target: AGLU (alpha-Glucosidase (AGLU))

Alternative Name: alpha-glucosidase (AGLU Products)

Synonyms: MG antibody, MGA antibody, SPAC56F8.01 antibody, SPAC922.02c antibody, CG11909 antibody, CT33098 antibody, Dmel\\CG11909 antibody, GB19017 antibody, 6030407P20Rik antibody, maltase-glucoamylase antibody, alpha-glucosidase (predicted) antibody, alpha-glucosidase antibody, glucosidase, alpha, acid antibody, target of brain insulin antibody, sucrase-isomaltase antibody, MGAM antibody, SPAC30D11.01c antibody, LMOf2365_0194 antibody, SPAC1039.11c antibody, Gaa antibody, tobi antibody, SI antibody, Hbg3 antibody, Mgam antibody

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Pathways: Cellular Glucan Metabolic Process