Secretory Component antibody (Membrane-Bound, Soluble) (HRP)

Catalog No. ABIN458765

Product Details anti-Secretory Component Antibody (HRP)

Target: Secretory Component

Binding Specificity: Membrane-Bound, Soluble

Reactivity: Rhesus Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Secretory Component antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Specificity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent of the concentration of the reactants and he sensitivity of the method. This product may also react with free and bound secretory component in many old-world monkeys, including Macaca Sylvanus, Cercopithecus, Cynomolgus, Baboon, and also with the secretory component of chimpanzees. In double radial immunodiffusion a reaction has been observed with free human secretory component.

Characteristics: Horseradish peroxidase-conjugated IgG fraction of polyclonal goat antiserum to rhesus monkey secretory component, free and bound

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Secretory component can be present in monkey secretions bound to secretory IgA (sIgA) and in free form. Free monkey secretory component isolated from pooled milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In enzyme-immunocytochemical and immunohistochemical staining of free or bound secretory component at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA, Western blotting) in monkey milk or other secretions. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:150 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:200 and 1:4,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. No foreign proteins added. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2)

Buffer: Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

References for anti-Secretory Component antibody (HRP) (ABIN458765)

Thomas, Tuero, Demberg, Vargas-Inchaustegui, Musich, Xiao, Venzon, LaBranche, Montefiori, DiPasquale, Reed, DeVico, Fouts, Lewis, Gallo, Robert-Guroff: "HIV-1 CD4-induced (CD4i) gp120 epitope vaccines promote B and T-cell responses that contribute to reduced viral loads in rhesus macaques." in: Virology, Vol. 471-473, pp. 81-92, (2015) (PubMed).

Target Details for Secretory Component

Target: Secretory Component

Abstract: Secretory Component Products

Synonyms: RNPIGR2 antibody, pIgA-R antibody, polymeric immunoglobulin receptor antibody, PIGR antibody, Pigr antibody

Background: Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with free secretory component. In immunoelectrophoresis against normal monkey milk, using a high electroendosmosis agar plate, free secretory component ism precipitated in the alpha-2 region. This antiserum does not react with other molecular forms of IgA, or with any other secretory or plasma protein and double radial immunodiffusion.