Complement C3c antibody

Catalog No. ABIN458503

Product Details anti-Complement C3c Antibody

Target: Complement C3c (C3C) (Complement Component C3c (C3C))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Complement C3c antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity: The antiserum does not cross-react with any other component of mouse plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Purified IgG fraction of polyclonal goat antiserum to C3c fragment of mouse complement factor C3

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: C3 is the most abundant complement protein in mouse serum. Its biological function strongly resembles that of C3 in man and other laboratory animal species. It has a central role in the activation system being common to both pathways. Activation of C3 is achieved by very specific limited proteolysis resulting in the release of a number of degradation fragments. The anaphylotoxin C3a promotes smooth muscle contraction and increases vascular permeability: the large C3b fragment is involved in binding to the complement activator and can be interact with specific receptors to allow efficient clearance of the activating cell or particle, degradation fragments of C3b (C3bi, C3c, C3dg C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possibly in the immune response. The antiserum is raised against C3c, which is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. Consequently the antiserum reacts with both native and activated C3. It may also react with the fragments C3b, C3bi and C3dg, since they all carry antigenic epitopes of the C3c domain. C3c is isolated and purified from pooled normal mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: As unlabelled primary or secondary antibody reagent for the indirect detection of C3c in mouse cells, tissues and body fluids in immunofluorescence and immunoenzyme methods, for the production of immunoconjugates with a selected marker, to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier, as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). Locally deposited immune complexes in tissue usually contain complement, pointing to activation of the classical pathway. Complement activation in vivo implies active disease and may contribute to the elicitation of the pathogenesis and he extent of tissue destruction. Sometimes the diagnosis can be based on directly on laboratory findings. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Typical working dilutions in histochemistry are usually between 1:50 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:2,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. No foreign proteins added. Antibody titre: Precipitin titre 1:16 when tested against pooled normal mouse serum in agar-block immunodiffusion titration.

Buffer: Purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

References for anti-Complement C3c antibody (ABIN458503)

Ermert, Shaughnessy, Joeris, Kaplan, Pang, Kurt-Jones, Rice, Ram, Blom: "Virulence of Group A Streptococci Is Enhanced by Human Complement Inhibitors." in: PLoS pathogens, Vol. 11, Issue 7, pp. e1005043, (2016) (PubMed).

Target Details for Complement C3c

Target: Complement C3c (C3C) (Complement Component C3c (C3C))

Alternative Name: C3c Fragment of Complement Factor C3 (C3C Products)

Background: In immunoelectrophoresis against fresh mouse serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other protein components of mouse serum or plasma