SERPIND1 antibody

Catalog No. ABIN458146

Product Details anti-SERPIND1 Antibody

Target: SERPIND1 (serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SERPIND1 antibody is un-conjugated

Application: Immunoelectrophoresis (IEP), Immunodiffusion (ID), Radial Immunodiffusion (RID)

Specificity: The antiserum does not cross react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Precipitating polyclonal rabbit antiserum to human heparin cofactor II

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Heparin cofactor II is a glycoprotein synthesized in the liver and has a molecular weight of 65,000. It is a plasma serine inhibitor of thrombin. Antithrombin III is more slowly inhibited. A hereditary form of inactive heparin cofactor II has been described. Reduced plasma values have been observed in disseminated intravascular coagulopathy and in lever disease. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of heparin cofactor II in human plasma or other body fluids or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Measured by quantitative precipitin analysis. The amount of heparin cofactor II precipitated by 1 ml an

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Target Details for SERPIND1

Target: SERPIND1 (serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))

Alternative Name: Heparin Cofactor II (SERPIND1 Products)

Synonyms: D22S673 antibody, HC2 antibody, HCF2 antibody, HCII antibody, HLS2 antibody, LS2 antibody, THPH10 antibody, serpind1 antibody, MGC53936 antibody, rls2var1 antibody, AA985900 antibody, AI303446 antibody, Hcf2 antibody, serpin family D member 1 antibody, serpin peptidase inhibitor, clade D (heparin cofactor), member 1 L homeolog antibody, serpin peptidase inhibitor, clade D (heparin cofactor), member 1 antibody, serine (or cysteine) peptidase inhibitor, clade D, member 1 antibody, SERPIND1 antibody, serpind1.L antibody, Serpind1 antibody, serpind1 antibody

Background: The reactivity of the antiserum is restricted to heparin cofactor II. In bidimensional electrophoresis against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified immunogen. No reaction is obtained with any other plasma protein component or serum