C1q antibody
C1q
Reactivity: Human
IEP
Host: Rabbit
Polyclonal
unconjugated
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- Target See all C1q Antibodies
- C1q (Complement Fragment C1q (C1q))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This C1q antibody is un-conjugated
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Application
- Immunoelectrophoresis (IEP)
- Specificity
- The antiserum does not cross react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.
- Characteristics
- Precipitating polyclonal rabbit antiserum to the C1q subunit of C1 component of human complement
- Purification
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
- Immunogen
- C1 component of human complement is a complex of three glycoproteins: C1q, C1r and C1s. C1q has a molecular weight of 492.000 and is present in plasma in an average concentration of 80 µ g/ml. It consists of a complex of 18 polypeptide chains of three different types, A, B and C, 6 of each type. The subcomponent C1q is responsible for the binding of C1q to immuno-complexes an membranes via the Fc portion of the immunoglobulin. The subunit C1q is isolated as a homogenous protein for use in antiserum production. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
- Top Product
- Discover our top product C1q Primary Antibody
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- Application Notes
- In precipitating techniques as immunoelectrophoresis and single or double radial immuno-diffusion to identify the presence of C1q or C1q containing complexes in human plasma or other body fluids or to determine its concentration. The presence of non precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive non-precipitating antibody-binding techniques if proper controls are included.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against in agar-block immunodiffusion titration against an appropriat
- Buffer
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
- Preservative
- Without preservative
- Storage
- 4 °C
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- Target
- C1q (Complement Fragment C1q (C1q))
- Alternative Name
- C1q Subunit of C1 Component of Complement (C1q Products)
- Synonyms
- AI255395 antibody, C1q antibody, complement component 1, q subcomponent, alpha polypeptide antibody, C1qa antibody
- Background
- The reactivity of the antiserum is restricted to C1q. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C1q protein. Complexes containing C1q may also react with the antiserum. No reaction is obtained with C1r, C1s or any other plasma protein component or serum
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