PRR7 antibody (C-Term, Intracellular)

Catalog No. ABIN457438

Product Details anti-PRR7 Antibody

Target: PRR7 (Proline Rich 7 (Synaptic) (PRR7))

Binding Specificity: C-Term, Intracellular

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRR7 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Specificity: The mouse monoclonal antibody TRAP3/10 recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex.

Cross-Reactivity (Details): Human, Mouse, Rat

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253)

Clone: TRAP3-10

Isotype: IgG2a

Application Details

Application Notes: Immunocytochemistry: Recommended dilution: 10 μg/mL, cell culture fixed with 4 % paraformaldehyde, permeabilized with 0.1 % Triton-X100.
Western blotting: Recommended dilution: 1 μg/mL, positive control: murine brain lysate (red. Laemmli buffer).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-PRR7 antibody (ABIN457438)

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).

Target Details for PRR7

Target: PRR7 (Proline Rich 7 (Synaptic) (PRR7))

Alternative Name: PRR7 / TRAP3 (PRR7 Products)

Synonyms: PRR7 antibody, XM_484263 antibody, proline rich 7, synaptic antibody, proline rich 7 (synaptic) antibody, proline rich 7 (synaptic) L homeolog antibody, PRR7 antibody, prr7 antibody, prr7.L antibody, Prr7 antibody

Background: Proline rich 7, synaptic,PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain). Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse.,TRAP3

Gene ID: 80758

UniProt: Q8TB68