ENPP3 antibody

Catalog No. ABIN457434

Product Details anti-ENPP3 Antibody

Target: ENPP3 (Ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENPP3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC)

Specificity: The mouse monoclonal antibody NP4D6 reacts with an extracellular epitope of CD203c, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: HEK-293 cells transfected with human CD203c

Clone: NP4D6

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL. Extracellular and intracellular staining.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-ENPP3 antibody (ABIN457434)

Heneberg, Riegerová, Kučera: "Pimecrolimus Is a Potent Inhibitor of Allergic Reactions to Hymenopteran Venom Extracts and Birch Pollen Allergen In Vitro." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142953, (2015) (PubMed).

Platz, Binder, Marxer, Lischka, Valent, Bühring: "Hymenoptera-venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/phosphodiesterase 3 in sensitized individuals." in: International archives of allergy and immunology, Vol. 126, Issue 4, pp. 335-42, (2002) (PubMed).

Target Details for ENPP3

Target: ENPP3 (Ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3))

Alternative Name: CD203c (ENPP3 Products)

Synonyms: B10 antibody, CD203c antibody, NPP3 antibody, PD-IBETA antibody, PDNP3 antibody, Npp3 antibody, RB13-6 antibody, AI876438 antibody, ENPP3 antibody, ectonucleotide pyrophosphatase/phosphodiesterase 3 antibody, ectonucleotide pyrophosphatase/phosphodiesterase 3 L homeolog antibody, ENPP3 antibody, Enpp3 antibody, enpp3 antibody, enpp3.L antibody

Background: Ectonucleotide pyrophosphatase/phosphodiesterase 3,CD203c, also known as ENPP-3, is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.,ENPP3, B10, PDNP3, PD-IBETA

Gene ID: 5169

UniProt: O14638

Pathways: Regulation of Muscle Cell Differentiation, Negative Regulation of Transporter Activity