CD81 antibody

Catalog No. ABIN457431

Product Details anti-CD81 Antibody

Target: CD81

Reactivity: Human, Rabbit, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD81 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Specificity: The antibody M38 reacts with an extracellular epitope of CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.

Cross-Reactivity (Details): Human, Feline (Cat), Rabbit

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: MOLT-4 (human T-ALL cell line)

Clone: M38

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: recommended dilution: 1 μg/mL.
Western blotting: recommended dilution: 1-2 μg/mL, positive control: Jurkat cells, non-reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD81 antibody (ABIN457431)

Oka, Kobayashi, Matsumura, Nishio, Nakano, Okamura, Okochi, Minamisawa, Shiba, Saeki: "New Role for Growth/Differentiation Factor 15 in the Survival of Transplanted Brown Adipose Tissues in Cooperation with Interleukin-6." in: Cells, Vol. 9, Issue 6, (2020) (PubMed).

Matsumura, Minamisawa, Suga, Kishita, Akagi, Ichiki, Ichikawa, Shiba: "Subtypes of tumour cell-derived small extracellular vesicles having differently externalized phosphatidylserine." in: Journal of extracellular vesicles, Vol. 8, Issue 1, pp. 1579541, (2019) (PubMed).

Escola, Kleijmeer, Stoorvogel, Griffith, Yoshie, Geuze: "Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes." in: The Journal of biological chemistry, Vol. 273, Issue 32, pp. 20121-7, (1998) (PubMed).

Imai, Kakizaki, Nishimura, Yoshie: "Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 3, pp. 1229-39, (1995) (PubMed).

Imai, Yoshie et al.: "C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 6470-81, (1994) (PubMed).

Fukudome, Furuse, Imai, Nishimura, Takagi, Hinuma, Yoshie et al.: "Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen ..." in: Journal of virology, Vol. 66, Issue 3, pp. 1394-401, (1992) (PubMed).

Target Details for CD81

Target: CD81

Alternative Name: CD81 (CD81 Products)

Synonyms: Tapa-1 antibody, Tapa1 antibody, Tspan28 antibody, CVID6 antibody, S5.7 antibody, TAPA1 antibody, TSPAN28 antibody, CD81 molecule antibody, CD81 molecule b antibody, CD81 antigen antibody, Cd81 molecule antibody, CD81 antibody, cd81b antibody, Cd81 antibody

Background: CD81 Molecule,CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells, e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus.,S5.7, CVID6, TAPA1, TSPAN28

Gene ID: 975

UniProt: P60033

Pathways: Inositol Metabolic Process, Hepatitis C