beta Catenin antibody
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- Target See all beta Catenin (CATNB) Antibodies
- beta Catenin (CATNB) (Catenin, beta (CATNB))
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Reactivity
- Human, Mouse, Hamster
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This beta Catenin antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Specificity
- The mouse monoclonal antibody EM-22 reacts with C-terminal part of beta-catenin (intracellular antigen), an 88 kDa multifunctional protein involved both in cell adhesion and in activation of transcription.
- Cross-Reactivity (Details)
- Human, Mouse, Hamster
- Purification
- Purified by protein-A affinity chromatography.
- Purity
- > 95 % (by SDS-PAGE)
- Immunogen
- Recombinant human beta-catenin
- Clone
- EM-22
- Isotype
- IgG2a
- Top Product
- Discover our top product CATNB Primary Antibody
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- Application Notes
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Immunocytochemistry: Positive control: HT29 human colon adenocarcinoma cell line.
Western blotting: Positive control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1 human colon adenocarcinoma cell line, KW1 murine cell line, C57MG murine cell line, 3T3 murine fibroblast cell line.
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining. - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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- Target
- beta Catenin (CATNB) (Catenin, beta (CATNB))
- Alternative Name
- beta-Catenin (CATNB Products)
- Synonyms
- Bfc antibody, Catnb antibody, Mesc antibody, CTNNB antibody, MRD19 antibody, armadillo antibody, ctnnb antibody, id:ibd2058 antibody, wu:fb73e10 antibody, wu:fi81c06 antibody, wu:fk25h01 antibody, beta-catenin antibody, ctnnb1 antibody, CHBCAT antibody, catenin (cadherin associated protein), beta 1 antibody, catenin beta 1 antibody, catenin (cadherin-associated protein), beta 1 antibody, catenin beta 1 L homeolog antibody, Ctnnb1 antibody, CTNNB1 antibody, ctnnb1 antibody, ctnnb1.L antibody
- Background
- Catenin beta 1,Beta-catenin is a multifunctional protein involved both in cell adhesion and in activation of transcription. Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound alpha, beta and gamma catenin. Beta-catenin links this complex through alpha-actinin to the cytoskeleton. Functional cadherin-catenin system is important for invasiveness of tumour cells. Beta-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 beta. When activity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway), hypophosphorylated stable form of beta-catenin accumulates in the cytoplasm, translocates to the nucleus and activates transcription of genes including those that are involved in cell cycle control. As a result, cell division and neoplastic transformation are promoted.,CTNNB1, EVR7, MRD19
- Gene ID
- 1499
- UniProt
- P35222
- Pathways
- Peptide Hormone Metabolism
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