L-Selectin antibody

Catalog No. ABIN457405

Product Details anti-L-Selectin Antibody

Target: L-Selectin (SELL) (Selectin L (SELL))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This L-Selectin antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The rat monoclonal antibody MEL-14 reacts with an extracellular epitope of murine CD62L (L-selectin), a 75 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes, it is also present on a subset of NK cells and certain hematopoietic malignant cells.

Cross-Reactivity (Details): Mouse

Purification: Purified by protein-G affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: C3H/eb mouse B cell lymphoma 38C-13

Clone: Mel-14

Isotype: IgG2a

Application Details

Application Notes: Functional application: Blocking of cell adhesion.
Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Preservative: Azide free

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-L-Selectin antibody (ABIN457405)

Xu, Manivannan, Crane, Dawson, Liversidge: "Critical but divergent roles for CD62L and CD44 in directing blood monocyte trafficking in vivo during inflammation." in: Blood, Vol. 112, Issue 4, pp. 1166-74, (2008) (PubMed).

Pop, Wong, He, Wang, Wallet, Goudy, Tisch: "The type and frequency of immunoregulatory CD4+ T-cells govern the efficacy of antigen-specific immunotherapy in nonobese diabetic mice." in: Diabetes, Vol. 56, Issue 5, pp. 1395-402, (2007) (PubMed).

Richards, Longhi, Wright, Gallimore, Ager: "CD62L (L-selectin) down-regulation does not affect memory T cell distribution but failure to shed compromises anti-viral immunity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 1, pp. 198-206, (2007) (PubMed).

Chen, Cui, Sempowski, Liu, Chao: "Transfer of allogeneic CD62L- memory T cells without graft-versus-host disease." in: Blood, Vol. 103, Issue 4, pp. 1534-41, (2004) (PubMed).

Chin, Miller, Graham, Parviz, Zacur, Patel, Duong, Bear: "Bryostatin 1/ionomycin (B/I) ex vivo stimulation preferentially activates L-selectinlow tumor-sensitized lymphocytes." in: International immunology, Vol. 16, Issue 9, pp. 1283-94, (2004) (PubMed).

Friedline, Wong, Steeber, Tedder, Tisch: "L-selectin is not required for T cell-mediated autoimmune diabetes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 6, pp. 2659-66, (2002) (PubMed).

Mannering, Cheers: "Interleukin-2 and loss of immunity in experimental Mycobacterium avium infection." in: Infection and immunity, Vol. 70, Issue 1, pp. 27-35, (2001) (PubMed).

ten Bruggencate, Hillyer, Woodward: "The proportion of CD45RA(+)CD62L(+) (quiescent-phenotype) T cells within the CD8(+) subset increases in advanced weight loss in the protein- or energy-deficient weanling mouse." in: The Journal of nutrition, Vol. 131, Issue 12, pp. 3266-9, (2001) (PubMed).

Brawand, Cerottini, MacDonald: "Hierarchal utilization of different T-cell receptor Vbeta gene segments in the CD8(+)-T-cell response to an immunodominant Moloney leukemia virus-encoded epitope in vivo." in: Journal of virology, Vol. 73, Issue 11, pp. 9161-9, (1999) (PubMed).

Target Details for L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Alternative Name: CD62L / L-Selectin (SELL Products)

Synonyms: CD62L antibody, LAM1 antibody, LECAM1 antibody, LEU8 antibody, LNHR antibody, LSEL antibody, LYAM1 antibody, PLNHR antibody, TQ1 antibody, A.11 antibody, L-selectin antibody, LECAM-1 antibody, SELE antibody, AI528707 antibody, Lnhr antibody, Ly-22 antibody, Ly-m22 antibody, Lyam-1 antibody, Lyam1 antibody, selectin L antibody, selectin, lymphocyte antibody, SELL antibody, Sell antibody

Background: Selectin L,CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).,L-selectin, Lsel, Sell, Lam1, Lecam, Leu8, Lnhr, Lyam1, Plnhr

Gene ID: 20343

UniProt: Q3TCF3