EpCAM antibody (FITC)

Catalog No. ABIN457357

Product Details anti-EpCAM Antibody (FITC)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody VU-1D9 recognizes an extracellular epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.

Cross-Reactivity (Details): Human, Other species Not tested

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Small cell lung carcinoma cell line H69.

Clone: VU-1D9

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-EpCAM antibody (FITC) (ABIN457357)

Brunner, Prelog, Verdorfer, Tzankov, Mikuz, Ensinger: "EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder." in: Journal of clinical pathology, Vol. 61, Issue 3, pp. 307-10, (2008) (PubMed).

Winter, Nagtegaal, van Krieken, Litvinov: "The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology." in: The American journal of pathology, Vol. 163, Issue 6, pp. 2139-48, (2003) (PubMed).

Li, Passebosc-Faure, Lambert, Gentil-Perret, Blanc, Oosterwijk, Mosnier, Genin, Tostain: "Flow cytometric analysis of antigen expression in malignant and normal renal cells." in: Anticancer research, Vol. 20, Issue 4, pp. 2773-8, (2000) (PubMed).

Ogura, Senzaki, Yoshizawa, Hioki, Tsubura: "Immunohistochemical localization of epithelial glycoprotein EGP-2 and carcinoembryonic antigen in normal colonic mucosa and colorectal tumors." in: Anticancer research, Vol. 18, Issue 5B, pp. 3669-75, (1999) (PubMed).

Tsubura, Senzaki, Sasaki, Hilgers, Morii: "Immunohistochemical demonstration of breast-derived and/or carcinoma-associated glycoproteins in normal skin appendages and their tumors." in: Journal of cutaneous pathology, Vol. 19, Issue 1, pp. 73-9, (1992) (PubMed).

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: CD326 / EpCAM (EPCAM Products)

Background: Epithelial cell adhesion molecule,CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.,EPCAM, GA733-2, EGP314, KSA, KS 1/4 antigen, Trop-1, M4S1, DIAR5, MIC18, TROP1

Gene ID: 4072

UniProt: P16422