ITGAL antibody (FITC)

Catalog No. ABIN457347

Product Details anti-ITGAL Antibody (FITC)

Target: ITGAL (Integrin alpha-L (ITGAL))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGAL antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: The rat monoclonal antibody M17/4 reacts with an extracellular epitope of CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.

Cross-Reactivity (Details): Mouse

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: C57BL/6 mouse splenic secondary cytotoxic T lymphocytes

Clone: M17-4

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-2 μg/mL.

Comment:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-ITGAL antibody (FITC) (ABIN457347)

Angelov, Guillaume, Cebecauer, Bosshard, Dojcinovic, Baumgaertner, Luescher: "Soluble MHC-peptide complexes containing long rigid linkers abolish CTL-mediated cytotoxicity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 6, pp. 3356-65, (2006) (PubMed).

Beyer, Wang, Peters, Doths, Koerner-Rettberg, Openshaw, Schwarze: "The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo." in: Respiratory research, Vol. 6, pp. 70, (2005) (PubMed).

Bellingan, Xu, Cooksley, Cauldwell, Shock, Bottoms, Haslett, Mutsaers, Laurent: "Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation." in: The Journal of experimental medicine, Vol. 196, Issue 11, pp. 1515-21, (2002) (PubMed).

Koseki, Miura, Fujimori, Hokari, Komoto, Hara, Ogino, Nagata, Goto, Hachimura, Kaminogawa, Ishii: "In situ demonstration of intraepithelial lymphocyte adhesion to villus microvessels of the small intestine." in: International immunology, Vol. 13, Issue 9, pp. 1165-74, (2001) (PubMed).

Papayannopoulou, Priestley, Nakamoto, Zafiropoulos, Scott, Harlan: "Synergistic mobilization of hemopoietic progenitor cells using concurrent beta1 and beta2 integrin blockade or beta2-deficient mice." in: Blood, Vol. 97, Issue 5, pp. 1282-8, (2001) (PubMed).

Kuhlman, Moy, Lollo, Brian: "The accessory function of murine intercellular adhesion molecule-1 in T lymphocyte activation. Contributions of adhesion and co-activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 6, pp. 1773-82, (1991) (PubMed).

Sanchez-Madrid, Davignon, Martz, Springer: "Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship." in: Cellular immunology, Vol. 73, Issue 1, pp. 1-11, (1983) (PubMed).

Target Details for ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: CD11a (ITGAL Products)

Synonyms: CD11A antibody, LFA-1 antibody, LFA1A antibody, (p180) antibody, Cd11a antibody, LFA-1A antibody, Ly-15 antibody, Ly-21 antibody, CD11a antibody, ITGAL antibody, integrin subunit alpha L antibody, integrin alpha L antibody, ITGAL antibody, Itgal antibody

Background: Integrin subunit alpha L,CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.,LFA-1, LFA1A, ITGAL

Gene ID: 16408

UniProt: E9Q5M7

Pathways: Activated T Cell Proliferation, Integrin Complex