Reactive with natural and recombinant human MCP-3. Does not show any cross reaction with other Human Cytokines or growth factors tested such as IL1 beta, IL-8, MCAF, SAA and EGF
Cross-Reactivity (Details)
Species reactivity (tested):Human.
Purification
Affinity Chromatography on Protein G.
Immunogen
Purified Recombinant Human Monocyte Chemotactic Protein-3 (MCP-3).
CCL7
Reactivity: Human
WB, ELISA
Host: Mouse
Polyclonal
unconjugated
Application Notes
ELISA: This MCP3 antibody Biotin-conjugated can be used as a Tracer/Detection Antibodyin Sandwich ELISA applications for human MCP-3 detection in combination with a Captureantibody (Cat#DM2039)Suggested Capture Coating Dose: 3 μg/wellSubstrate: TMB Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Macrophage/monocyte chemotactic protein-3 (MCP-3), like other two closely related chemokines (MCP1 and MCP-2), is chemotactic to monocytes, but not to neutrophils. MCP-3 showed 78 % sequence homology with MCP-1 and 58 % sequence homology with MCP-2. The primary receptor for MCP-3 is D6. MCP-3 also competes with MCP-1 to bind to CCR10, and competes with Rantes and MIP-1α to bind to CCR1. MCP-3 stimulates macrophage to secrete proteases. A variety of cancer cell lines express MCP-3, suggesting a role in tumor invasion and metastasia.Synonyms: C-C motif chemokine 7, CCL-7, MCP-3, Macrophage Chemotactic Protein-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, SCYA6, SCYA7, Small-inducible cytokine A7