DLC1
Reactivity: Human, Mouse, Rat
IHC, ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Peptide ELISA: Antibody Detection Limit Dilution: 1/2,000. Western Blot: Preliminary experiments gave an approx 28 kDa band in Human Liver andLung lysates as well as in different epithelial lung cell lines after 0.3 μg/mL antibodystaining. Please note that currently we cannot find an explanation in the literature for theband we observe given the calculated size of 171 kDa according to NP_872584.1 and54.8 kDa according to NP_079043.2 (isotope 3). The 28 kDa band was successfully blockedby incubation with the immunizing peptide. Immunohistochemistry: 5 μg/mL. In paraffin embedded Human Adrenal Gland (Medulla)shows strong reticulate staining in the cytoplasm of medular cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
ARHGAP7 antibody, HP antibody, STARD12 antibody, p122-RhoGAP antibody, rhogap7 antibody, DLC-1 antibody, StARD12 antibody, Dlc-1 antibody, A730069N07Rik antibody, Arhgap7 antibody, dlc-1 antibody, RhoGAP antibody, Dlc1 antibody, DLC1 Rho GTPase activating protein antibody, DLC1 Rho GTPase activating protein S homeolog antibody, rho GTPase-activating protein 7 antibody, deleted in liver cancer 1 antibody, DLC1 antibody, dlc1.S antibody, dlc1 antibody, LOC100456847 antibody, Dlc1 antibody, LOC100729904 antibody
Background
DLC1 functions as a GTPase-activating protein specific for Rho and an activator of PLCD1 in vivo and induces morphological changes and detachment through cytoskeletal reorganization. DLC1 is a candidate tumor suppressor gene for human liver cancer, as well as for prostate, lung, colorectal, and breast cancers.Synonyms: Deleted in liver cancer 1 protein, HP protein, KIAA1723, Rho GTPase-activating protein 7, Rho-type GTPase-activating protein 7, STARD12, START domain-containing protein 12, StAR-related lipid transfer protein 12