HCV Core Protein antibody

Catalog No. ABIN452510

Product Details anti-HCV Core Protein Antibody

Target: HCV Core Protein (HCV C) (Hepatitis C Virus Core Protein (HCV C))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCV Core Protein antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: Hepatitis C Virus

Purification: Affinity chromatography on Protein G.

Immunogen: Synthetic peptides derived from HCV Capsid Protein

Clone: A1-3D1

Isotype: IgG1

Application Details

Application Notes: ELISA: HCV Core Protein antibody (clone A1/3D1) was tested using indirect ELISA method. The testing plates were coated with HCV non-structural protein (NS), Capsid Protein (C) andthe mixture of NS and C separately. The neat culture supernatant of hybridoma showed a strong reaction with HCV capsidprotein (OD492 value > 2.0) and mixture of NS and C (OD492 value >1.5), and have noreaction with HCV Non-structural protein (OD492 value < 0.03). Not suitable for use in Western Blots. Has been used successfully in the recognition of in-vitro translated HCV core protein.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL

Buffer: 0.01 M PBS, pH 7.0 without preservatives.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for HCV Core Protein

Target: HCV Core Protein (HCV C) (Hepatitis C Virus Core Protein (HCV C))

Alternative Name: HCV Core Protein (HCV C Products)

Target Type: Viral Protein

Background: The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein, it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR. Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Hepatitis C virus (HCV) causes most cases of non-A, non-B hepatitis and results in most HCV infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. T cell responses, including interferon-gamma production are severely suppressed in chronic HCV patients.Synonyms: Hepatitis C Virus core protein