Peptide ELISA: 1/8000. Immunohistochemistry on paraffin sections: 3 - 5 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0,5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Target
PIAS2
(Protein Inhibitor of Activated STAT, 2 (PIAS2))
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Background
PIAS2 functions as an E3 type small ubiquitin like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO mediated repression of ELK1 transactivation activity. Isoform PIAS2 beta, but not isoform PIAS2 alpha, promotes MDM2 sumoylation. Isoform PIAS2 beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2 alpha.Synonyms: ARIP3, Androgen receptor-interacting protein 3, DAB2-interacting protein, DIP, E3 SUMO-protein ligase PIAS2, Miz1, Msx-interacting zinc finger protein, PIAS-NY protein, PIASX, Protein inhibitor of activated STAT x, Protein inhibitor of activated STAT2