Recombinant Blue Fluorescent Protein antibody (Atto 488)

Catalog No. ABIN4368359

Product Details anti-Blue Fluorescent Protein Antibody (Atto 488)

Target: Blue Fluorescent Protein (BFP)

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Entacmaea quadricolor

Host: Alpaca

Expression System: E.coli

Clonality: Monoclonal

Conjugate: This Blue Fluorescent Protein antibody is conjugated to Atto 488

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Purpose: Camelid sdAb anti-TagFP conjugated with Atto488, Clone 1H7

Specificity: Recognizes mTagBFP, mKate, mKate2, mTagRFP, mTagRFP657 and most common fluorescent proteins deriving from Entacmaea quadricolor

Cross-Reactivity (Details): Does not cross-react with common GFP- or dsRed derivatives.

Characteristics: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Purification: Produced in: E.coli

Immunogen: BFP

Clone: 1H7

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Application Details

Application Notes: Recommended dilution 1:500

Comment:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 5 μM

Buffer: 2.5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light!

Storage: -20 °C,-80 °C

Storage Comment: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Expiry Date: 12 months

Target Details for Blue Fluorescent Protein

Target: Blue Fluorescent Protein (BFP)

Alternative Name: TagBFP (BFP Products)

Molecular Weight: 26 kDa