Recombinant Blue Fluorescent Protein antibody (Atto 488)
-
- Target See all Blue Fluorescent Protein (BFP) products
- Blue Fluorescent Protein (BFP)
- Antibody Type
- Recombinant Antibody
- Fragment
- single-domain Antibody (sdAb)
- Reactivity
- Entacmaea quadricolor
-
Host
-
Alpaca
- Expression System
- E.coli
-
Clonality
- Monoclonal
-
Conjugate
- This Blue Fluorescent Protein antibody is conjugated to Atto 488
- Application
- Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Purpose
- Camelid sdAb anti-TagFP conjugated with Atto488, Clone 1H7
- Specificity
- Recognizes mTagBFP, mKate, mKate2, mTagRFP, mTagRFP657 and most common fluorescent proteins deriving from Entacmaea quadricolor
- Cross-Reactivity (Details)
- Does not cross-react with common GFP- or dsRed derivatives.
- Characteristics
- A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
- Purification
- Produced in: E.coli
- Immunogen
- BFP
- Clone
- 1H7
- Labeling Ratio
- a single fluorophore is coupled to exactly one sdAb
-
anti-Blue Fluorescent Protein (BFP) antibody (Atto 488)
BFP Reactivity: Entacmaea quadricolor FACS, ICC, IF Host: Alpaca Monoclonal 1H7 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
-
- Application Notes
- Recommended dilution 1:500
- Comment
-
Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Concentration
- 5 μM
- Buffer
- 5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light!
- Storage
- -20 °C,-80 °C
- Storage Comment
- Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
- Expiry Date
- 12 months
-
- Target
- Blue Fluorescent Protein (BFP)
- Alternative Name
- TagBFP (BFP Products)
- Molecular Weight
- 26 kDa
-
