PRMT5 antibody (Internal Region)

Catalog No. ABIN401522

Product Details anti-PRMT5 Antibody

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This PRMT5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: RACREKDRDP EAQ

Specificity: This antibody detects PRMT5 (Internal). It is expected to recognize both isoforms (NP_006100.2, NP_001034708.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Pig, Dog, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_006100.2, NP_001034708.1. Genename: PRMT5

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 16000. Western blot: 1-3 μg/mL. Approx 70 kDa band observed in Jurkat lysates (calculated MW of72.0 kDa and 71.0 kDa according to NP_006100.2, NP_001034708.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for PRMT5

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Alternative Name: PRMT5 (PRMT5 Products)

Background: Arginine methylation is an irreversible post translational modification linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These proteins include nuclear proteins (Histone 2A, 3, 4), IL enhancer binding factor, nuclear factors (NF45, 90, ILF3, Nucleolin, STAT1, Poly(A) binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.Synonyms: 72 kDa ICln-binding protein, HRMT1L5, Histone-arginine N-methyltransferase PRMT5, IBP72, JBP1, Jak-binding protein 1, Protein Arginine N-Methyltransferase 5, SKB1, SKB1Hs

Gene ID: 10419

NCBI Accession: NP_001034708

UniProt: O14744

Pathways: Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development