ATR antibody (Internal Region)

Catalog No. ABIN401401

This Rabbit Polyclonal antibody specifically detects ATR in WB and EIA. It exhibits reactivity toward Human, Mouse, Dog, Fish, Monkey, Rat and Xenopus laevis.

Quick Overview for ATR antibody (Internal Region) (ABIN401401)

Target: ATR (Ataxia Telangiectasia and Rad3 Related (ATR))

Reactivity: Human, Mouse, Dog, Fish, Monkey, Rat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATR antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ATR Antibody

Binding Specificity: Internal Region

Specificity: This antibody is specific for ATR protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Monkey, Canine, Fish, Xenopus.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to an internal region of human ATR protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/15,000 - 1/70,000. Western Blot: 1/1,000 - 1/5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.28 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for ATR

Target: ATR (Ataxia Telangiectasia and Rad3 Related (ATR))

Alternative Name: ATR / FRP1

Background: Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidylinositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR activates checkpoint signaling upon genotoxic stresses, such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR is a member of the DNA-PK kinase family and is closely related to ATM and DNAPK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes, including histone deacetylases (HDAC1, HDAC2 and CHD4). ATR is known to phosphorylate BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53 which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR is a nuclear protein, but can also be found in PML nuclear bodies in certain cell types. ATR is recruited to chromatin during Sphase and redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.Synonyms: Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1, Serine/threonine-protein kinase ATR

Gene ID: 545

NCBI Accession: NP_001175

UniProt: Q13535

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus