RAB11FIP3
Reactivity: Human
Host: Rabbit
Polyclonal
Biotin
Application Notes
ELISA: 1/1,000 - 1/3,000. Western Blot: 1/200 - 1/2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.19 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Target
RAB11FIP3
(RAB11 Family Interacting Protein 3 (Class II) (RAB11FIP3))
Rab11 Family-Interacting Protein 3 (FIP3)/Arfophilin-1 and FIP4/Arfophilin-2 are dual effectors for Rab11 and the ADP-ribosylation factor Arf6. Rab11 and Arf6 are involved in membrane delivery from recycling endosomes to the plasma membrane during cytokinesis. The dual Rab11/Arf6 binding proteins, FIP3 and FIP4, function in the delivery of recycling endosomes to the cleavage furrow and are, together with Rab11, essential for completion of abscission, the terminal step of cytokinesis. An interaction between Rab11-FIP3 and ASAP1 has also been identified (personal communication, P. Randazzo, CCR-NCI, Bethesda, MD). ASAP1 is an Arf GTPase-activating protein that has been implicated in the invasive properties of uveal melanoma and mammary carcinoma.Synonyms: ARFO1, Arfophilin-1, Eferin, FIP3-Rab11, KIAA0665, MU-MB-17.148, Rab11 family-interacting protein 3, Rab11-FIP3