LIM Domain Kinase 1 antibody (AA 630-647)
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- Target See all LIM Domain Kinase 1 (LIMK1) Antibodies
- LIM Domain Kinase 1 (LIMK1)
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Binding Specificity
- AA 630-647
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Reactivity
- Human, Mouse, Rat, Chimpanzee, Orang-Utan
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This LIM Domain Kinase 1 antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificity
- This antibody reacts to LIM kinase protein.
- Cross-Reactivity (Details)
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Species reactivity (expected):Chimpanzee, Orangutan, Mouse, Rat.
Species reactivity (tested):Human. - Purification
- Affinity Purified
- Immunogen
- Synthetic peptide corresponding aa 630-647 of human LIM kinase protein
- Isotype
- IgG
- Top Product
- Discover our top product LIMK1 Primary Antibody
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- Application Notes
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ELISA: 1/8000 - 1/36000. Western Blot: 1/500 - 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
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- Target
- LIM Domain Kinase 1 (LIMK1)
- Alternative Name
- LIMK1 (LIMK1 Products)
- Synonyms
- limk1 antibody, xlimk1 antibody, GB16654 antibody, LIMK1 antibody, LIMK antibody, LIMK-1 antibody, LIM domain kinase 1 antibody, LIM domain kinase 1a antibody, LIM domain kinase 1 L homeolog antibody, LIM-domain containing, protein kinase antibody, LIMK1 antibody, limk1a antibody, limk1 antibody, CpipJ_CPIJ000717 antibody, LOC413152 antibody, Limk1 antibody, limk1.L antibody
- Background
- LIM Kinase is also known as LIM-domain containing protein kinase, LIMK-1 and LIMK. There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase 1 and LIM kinase 2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Two splice variant have been identified. LIM kinase has a cytoplamic localization.Synonyms: EC=2.7.11.1, LIM domain kinase 1, LIMK, LIMK-1
- Gene ID
- 3984
- NCBI Accession
- NP_001191355
- UniProt
- P53667
- Pathways
- Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events
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