Reactivity occurs against Human HR23B protein. Cross reactivity with HR23B protein from Mouse and Rat may also occur as sequence homology varies by one amino acid residue in this sequence by BLAST analysis. Reactivity with HR23B protein from other sources is not known.
Cross-Reactivity (Details)
Species reactivity (expected):Canine, Chimpanzee, S.cerevisiae. Species reactivity (tested):Human.
Purification
Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase
Immunogen
Synthetic peptide corresponding aa 163-176 of human HR23B protein
RAD23B
Reactivity: Human, Mouse, Rat
WB, ELISA, IHC (p), IF (p), ICC, IF (cc), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: 1/2000 - 1/10000. Western Blot: 1/500 - 1/2000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.1 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
HR23B (also known as UV excision repair protein RAD23 homolog B, XP-C repair complementing complex 58 kDa protein and p58) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.Synonyms: HR23B, UV excision repair protein RAD23 homolog B, XP-C repair-complementing complex 58 kDa protein