ELISA: 1/10,000 - 1/50,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 1/500 - 1/2,500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 0.1 mL of deionized water (or equivalent).
Concentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative (Do NOT add Sodium Azide!)
Preservative
Gentamicin sulfate
Precaution of Use
This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
IL-32A (also known as Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL32a, Interleukin-32 and IL-32 isoform 4) is a member of the cytokine family. IL-32a is a secreted protein selectively expressed in lymphocytes and plays a role in innate and adaptive immune responses. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFa and IL-8. It induces typical cytokine pathways of NF-kB and p38 MAPK. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.Synonyms: IL-32, NK4, Natural killer cells protein 4, TAIF, Tumor necrosis factor alpha-inducing factor