TP53BP2 antibody

Catalog No. ABIN401045

Product Details anti-TP53BP2 Antibody

Target: TP53BP2 (Tumor Protein P53 Binding Protein 2 (TP53BP2))

Reactivity: Human, Dog, Macaque

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TP53BP2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against ASPP2.

Cross-Reactivity (Details): Species reactivity (expected):Canine (100 %), Macaque (100 %), Mouse (80 %), Rat (80 %).
Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Immunogen: Synthetic peptide corresponding to an internal sequence of Human ASPP2 . Genename: ASPP2

Isotype: IgG

Application Details

Application Notes: ELISA: 1/2,500-1/10,000. Western Blot: 1/500-1/3,000. Expect a band approximately 125 kDa in size correspondingto ASPP2 in the appropriate cell lysate or extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 2.4 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. Dilute only prior to immediate use.

Target Details for TP53BP2

Target: TP53BP2 (Tumor Protein P53 Binding Protein 2 (TP53BP2))

Alternative Name: TP53BP2 (TP53BP2 Products)

Background: ASPP (ankyrinrepeat-, SH3-domain- proline-rich-region protein) proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, iASPP functionally inactivates p53. ASPPs may also regulate p63-and p73-mediated apoptosis. Both ASPP1 and 2 directly interact with p53 and specifically enhance the apoptotic function of p53 by stimulating its DNA binding and transactivation function on promoters of pro-apoptotic genes, such as Bax and PIG-3. Not all cell cycle arrest genes are affected, such as p21. Interestingly, expression of ASPP is frequently down-regulated in human breast carcinomas expressing wild-type p53 but not mutant p53. Therefore, ASPP might regulate the tumor suppression function of p53 in vivo.Synonyms: 53BP2, ASPP2, Apoptosis-stimulating of p53 protein 2, BBP, Bcl2-binding protein, NY-REN-51, Tumor suppressor p53-binding protein 2, p53-binding protein 2, p53BP2

Gene ID: 7159

NCBI Accession: NP_001026855

UniProt: Q13625