Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
Specificity
This antibody is directed against PMS2-134 and reacts with full length version of PMS2. The epitope was putatively mapped to amino acids 58-81 of human PMS2. Expect a band approximately 96 kDa in size corresponding to human PMS2 by western blotting in most cell lines and tissues as PMS2 is ubiquitously expressed.
Cross-Reactivity (Details)
Species reactivity (expected):Mouse, Rat, Chimpanzee (100 %)Species reactivity (tested):Human, Hamster
Characteristics
Synonyms: PMSL2, Mismatch repair endonuclease PMS2, PMS1 protein homolog 2
Purification
Protein chromatography
Immunogen
Recombinant human PMS2 corresponding to the first 133 amino acid residues of theprotein
PMS2
Reactivity: Human
ELISA, IF
Host: Mouse
Monoclonal
4A8
unconjugated
Application Notes
ELISA: 1: 5,000 - 1: 20,000. Western blot: 1: 500 - 1: 2,000. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.38 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 a buffer and 0.09 % (w/v) Sodium Azide as preservative
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C forlonger. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Shelf life: one year from despatch.
Expiry Date
12 months
Target
PMS2
(PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))
PMS2 is a highly conserved nuclear protein involved in mismatch repair during DNA replication and has been identified to be composed as a heterodimer of PMS2 and MLH1. PMS is part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4), Turcot syndrome (an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas) and supratentorial primitive neuroectodermal tumors with cafe-au-lait spots (SNTCL). The human PMS2 gene encodes an 862 aa, 96 kDa polypeptide.Synonyms: DNA mismatch repair protein PMS2, Mismatch repair endonuclease PMS2, PMS1 protein homolog 2, PMSL2