Monoclonal antibody X9A9 (#AM03215PU-N) recognizes Axonin-1 or Contactin-2, an axon-associated cell adhesion molecule that is attached to the neuronal membrane by a GPI-anchor. Tissue Sections: Retina, developing Neurons.
CNTN2
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Immunohistochemistry on Frozen Sections: 1 μg/mL (1/400). Recommended Positive Control: Chicken embryo Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in 0.5 mL water (gives 0.4 mg/mL stock solution).
Concentration
0.4 mg/mL
Buffer
PBS, pH 7.2 containing 5 mg/mL BSA as stabilizer and 0.05 % Kathon as preservative.
Preservative
Kathon CG
Precaution of Use
This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store lyophilized product at 2-8 °C. Store stock solution or aliquots thereof at -20 °C. Avoid repeated thawing and freezing. Shelf life: One year from despatch.
Axonin-1 is a cell adhesion molecule of the immunoglobulin superfamily that was shown to be an axon guidance cue in the central nervous system in vivo. Secreted axonin-1, which is structurally highly similar to membrane-bound axonin-1, is not derived from the cell surface but directly from an iternal pool. Axonin-1 contains 6 immunoglobulin-like domains and 4 fibronectin type-III domains.Synonyms: Axonin-1, CNTN2, TAG-1