CBP Tag antibody

Catalog No. ABIN398464

Product Details anti-CBP Tag Antibody

Target: CBP Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBP Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Sequence: KRRWKKNFIA VSAA

Specificity: CBP-tag Antibody recognizes either internal or C-terminal CBP tagged fusion proteins.

Purification: Immunoaffinity chromatography

Immunogen: A synthetic CBP tag peptide KRRWKKNFIAVSAANRFKKISSSGAL conjugated - KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL Western blot:0.1-1.0 µg/mL Western Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane. Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-CBP Tag antibody (ABIN398464)

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Park, Ménétret, Gumbart, Ludtke, Li, Whynot, Rapoport, Akey: "Structure of the SecY channel during initiation of protein translocation." in: Nature, Vol. 506, Issue 7486, pp. 102-6, (2014) (PubMed).

Zid, OShea: "Promoter sequences direct cytoplasmic localization and translation of mRNAs during starvation in yeast." in: Nature, Vol. 514, Issue 7520, pp. 117-21, (2014) (PubMed).

Szklarczyk, Wanschers, Nijtmans, Rodenburg, Zschocke, Dikow, van den Brand, Hendriks-Franssen, Gilissen, Veltman, Nooteboom, Koopman, Willems, Smeitink, Huynen, van den Heuvel: "A mutation in the FAM36A gene, the human ortholog of COX20, impairs cytochrome c oxidase assembly and is associated with ataxia and muscle hypotonia." in: Human molecular genetics, Vol. 22, Issue 4, pp. 656-67, (2013) (PubMed).

van Bon, Oortveld, Nijtmans, Fenckova, Nijhof, Besseling, Vos, Kramer, de Leeuw, Castells-Nobau, Asztalos, Viragh, Ruiter, Hofmann, Eshuis, Collavin, Huynen, Asztalos, Verstreken, Rodenburg, Smeitink et al.: "CEP89 is required for mitochondrial metabolism and neuronal function in man and fly. ..." in: Human molecular genetics, Vol. 22, Issue 15, pp. 3138-51, (2013) (PubMed).

Cestari, Kalidas, Monnerat, Anupama, Phillips, Stuart: "A multiple aminoacyl-tRNA synthetase complex that enhances tRNA-aminoacylation in African trypanosomes." in: Molecular and cellular biology, Vol. 33, Issue 24, pp. 4872-88, (2013) (PubMed).

Thivierge, Makil, Flamand, Vasale, Mello, Wohlschlegel, Conte, Duchaine: "Tudor domain ERI-5 tethers an RNA-dependent RNA polymerase to DCR-1 to potentiate endo-RNAi." in: Nature structural & molecular biology, Vol. 19, Issue 1, pp. 90-7, (2012) (PubMed).

Wanschers, Szklarczyk, Pajak, van den Brand, Gloerich, Rodenburg, Lightowlers, Nijtmans, Huynen: "C7orf30 specifically associates with the large subunit of the mitochondrial ribosome and is involved in translation." in: Nucleic acids research, Vol. 40, Issue 9, pp. 4040-51, (2012) (PubMed).

Carnes, Schnaufer, McDermott, Domingo, Proff, Steinberg, Kurtz, Stuart: "Mutational analysis of Trypanosoma brucei editosome proteins KREPB4 and KREPB5 reveals domains critical for function." in: RNA (New York, N.Y.), Vol. 18, Issue 10, pp. 1897-909, (2012) (PubMed).

Woods, Mesquita, Sweet, Carvalho, Li, Liu, Nguyen, Thomas, Iversen, Marsillac, Karchin, Koomen, Monteiro: "Charting the landscape of tandem BRCT domain-mediated protein interactions." in: Science signaling, Vol. 5, Issue 242, pp. rs6, (2012) (PubMed).

Ringpis, Lathrop, Aphasizhev: "iCODA: RNAi-based inducible knock-in system in Trypanosoma brucei." in: Methods in molecular biology (Clifton, N.J.), Vol. 718, pp. 23-37, (2011) (PubMed).

Yu, Srinivasan, Nakanishi, Leatherwood, Shilatifard, Sternglanz: "A conserved patch near the C terminus of histone H4 is required for genome stability in budding yeast." in: Molecular and cellular biology, Vol. 31, Issue 11, pp. 2311-25, (2011) (PubMed).

Aphasizheva, Maslov, Wang, Huang, Aphasizhev: "Pentatricopeptide repeat proteins stimulate mRNA adenylation/uridylation to activate mitochondrial translation in trypanosomes." in: Molecular cell, Vol. 42, Issue 1, pp. 106-17, (2011) (PubMed).

Park, Rapoport: "Preserving the membrane barrier for small molecules during bacterial protein translocation." in: Nature, Vol. 473, Issue 7346, pp. 239-42, (2011) (PubMed).

Shin, Wolgamott, Yu, Blenis, Yoon: "Glycogen synthase kinase (GSK)-3 promotes p70 ribosomal protein S6 kinase (p70S6K) activity and cell proliferation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. E1204-13, (2011) (PubMed).

Target Details for CBP Tag

Target: CBP Tag

Alternative Name: CBP-Tag (CBP Tag Products)

Target Type: Tag

Background: Well-characterized antibodies consisting of short sequences have been widely and effectively used in the study of protein expression in various systems.CBP tag is a calmodulin-binding peptide, KRRWKKNFIAVSAANRFKKISSSGAL, derived from skeletal/cardiac muscle myosin light chain kinase 2.Rabbit Anti-CBP-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic CBP tag peptide conjugated to KLH.CBP-tag Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. The antibody is suitable for detecting CBP fusion proteins.