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S-Tag antibody

ELISA, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN398447
  • Target See all S-Tag products
    S-Tag
    Reactivity
    Please inquire
    Host
    • 27
    • 15
    • 1
    Rabbit
    Clonality
    • 27
    • 16
    Polyclonal
    Conjugate
    • 17
    • 6
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This S-Tag antibody is un-conjugated
    Application
    • 23
    • 18
    • 12
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB)
    Sequence
    KETAAAKFER QHMDS
    Specificity
    Rabbit Anti-S-tag Polyclonal Antibody specifically recognizes C-terminal, N-terminal, and internal S tagged fusion proteins.
    Purification
    Immunoaffinity chromatography
    Immunogen
    S epitope tag peptide KETAAAKFERQHMDS conjugated - KLH
    Isotype
    IgG
  • Application Notes
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    ELISA: 0.05-0.2 µg/mL
    Western blot: 0.1-1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit:For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Other applications: user-optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4, containing 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Storage
    4 °C/-20 °C
    Storage Comment
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Target
    S-Tag
    Abstract
    S-Tag Products
    Target Type
    Tag
    Background
    Rabbit Anti-S-tag Polyclonal Antibody is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide. It is purified by immunoaffinity chromatography.
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