MBP Tag antibody

Catalog No. ABIN398420

Product Details

Target: MBP Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: MBP-tag Antibody, mAb, Mouse specifically reacts with fusion proteins containing MBP epitope tags.

Purification: Protein A affinity column

Immunogen: Purified recombinant MBP fusion protein

Clone: 5B10B11

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.1-1.0 µg/mL
Western blot: 1.0 µg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details

Target: MBP Tag

Alternative Name: MBP-tag

Target Type: Tag

Background: Mouse Anti-MBP-tag Monoclonal Antibody reacts with recombinant maltose binding protein. The immunogen used to produce this B10 antibody is recombinant-MBP-epitope tagged fusion protein produced in E. coli.