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MBP Tag antibody

WB, ELISA Host: Mouse Monoclonal 5B10B11 unconjugated
Catalog No. ABIN398420
  • Target
    MBP Tag
    Host
    • 12
    • 5
    Mouse
    Clonality
    • 11
    • 5
    Monoclonal
    Conjugate
    • 15
    • 1
    • 1
    Un-conjugated
    Application
    • 15
    • 8
    • 6
    • 5
    • 3
    • 1
    • 1
    Western Blotting (WB), ELISA
    Specificity
    MBP-tag Antibody, mAb, Mouse specifically reacts with fusion proteins containing MBP epitope tags.
    Purification
    Protein A affinity column
    Immunogen
    Purified recombinant MBP fusion protein
    Clone
    5B10B11
    Isotype
    IgG
  • Application Notes
    Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    ELISA: 0.1-1.0 µg/mL
    Western blot: 1.0 µg/mL
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4, containing 0.02 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Storage
    4 °C/-20 °C
    Storage Comment
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Target
    MBP Tag
    Alternative Name
    MBP-tag
    Target Type
    Tag
    Background
    Mouse Anti-MBP-tag Monoclonal Antibody reacts with recombinant maltose binding protein. The immunogen used to produce this B10 antibody is recombinant-MBP-epitope tagged fusion protein produced in E. coli.
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