DDB1 antibody

Catalog No. ABIN396934

Product Details anti-DDB1 Antibody

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DDB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Brand: IHC-plus™

Specificity: Specific for the ~130kD human DDB1 protein. Reactivity has been confirmed with human HeLa cells.

Purification: Protein A purified

Immunogen: Recombinant human DDB1 protein.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (5 μg/mL), IP, WB (1 - 5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunoprecipitation: 5-10 μg/reaction. Positive control: Human HeLa cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for DDB1

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Alternative Name: DDB1 (DDB1 Products)

Synonyms: DDBA antibody, UV-DDB1 antibody, XAP1 antibody, XPCE antibody, XPE antibody, XPE-BF antibody, DDB1 antibody, xpe antibody, ddba antibody, xap1 antibody, xpce antibody, xpe-bf antibody, uv-ddb1 antibody, ddb1 antibody, 127kDa antibody, AA408517 antibody, p127-Ddb1 antibody, damage specific DNA binding protein 1 antibody, damage-specific DNA binding protein 1, 127kDa antibody, DNA damage-binding protein 1 antibody, damage-specific DNA binding protein 1 antibody, DDB1 antibody, ddb1 antibody, LOC100187356 antibody, Ddb1 antibody, ddb-1 antibody

Background: Name/Gene ID: DDB1

Synonyms: DDB1, DDB p127 subunit, DDBA, HBV X-associated protein 1, UV-DDB1, XAP-1, XPE, XPE-binding factor, UV-damaged DNA-binding factor, XPCE, DNA damage-binding protein 1, DNA damage-binding protein a, UV-DDB 1, XAP1, XPE-BF

Gene ID: 1642

UniProt: Q16531

Pathways: DNA Damage Repair