P4HB antibody (AA 499-509)

Catalog No. ABIN396933

Product Details anti-P4HB Antibody

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Binding Specificity: AA 499-509

Reactivity: Human, Mouse, Rat, Hamster, Cow, Dog, Guinea Pig, Monkey, Pig, Sheep, Rabbit, Xenopus laevis, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This P4HB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Recognizes rat protein disulfide isomerase at ~58kD.

Purification: Protein G purified

Immunogen: Synthetic peptide corresponding to aa499-509 of rat protein disulfide isomerase (KLH coupled).

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: HeLa Heat Shocked Cell Lysate, MCF-7 cell lysate.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: PDI / P4HB (P4HB Products)

Background: Name/Gene ID: P4HB

Synonyms: P4HB, ERBA2L, DSI, GIT, p4HB, p4Hbeta, PDIA1, PHDB, PO4DB, PROHB, Protocollagen hydroxylase, Protein disulfide-isomerase, PO4HB, PDI

Gene ID: 5034

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism