PFKFB4 antibody (AA 266-296)

Catalog No. ABIN392786

Product Details anti-PFKFB4 Antibody

Target: PFKFB4 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 4 (PFKFB4))

Binding Specificity: AA 266-296

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKFB4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: Pr

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PFKFB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-296 amino acids from the Central region of human PFKFB4.

Clone: RB04059

Isotype: IgG

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:100. IHC-P: 1:250

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PFKFB4 antibody (ABIN392786)

Ros, Flöter, Kaymak, Da Costa, Houddane, Dubuis, Griffiths, Mitter, Walz, Blake, Behrens, Brindle, Zamboni, Rider, Schulze: "6-Phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 is essential for p53-null cancer cells." in: Oncogene, Vol. 36, Issue 23, pp. 3287-3299, (2017) (PubMed).

Gómez, Manzano, Figueras, Viñals, Ventura, Rosa, Bartrons, Navarro-Sabaté: "Sertoli-secreted FGF-2 induces PFKFB4 isozyme expression in mouse spermatogenic cells by activation of the MEK/ERK/CREB pathway." in: American journal of physiology. Endocrinology and metabolism, Vol. 303, Issue 6, pp. E695-707, (2012) (PubMed).

Ros, Santos, Moco, Baenke, Kelly, Howell, Zamboni, Schulze: "Functional metabolic screen identifies 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 as an important regulator of prostate cancer cell survival." in: Cancer discovery, Vol. 2, Issue 4, pp. 328-43, (2012) (PubMed).

Yalcin, Clem, Simmons, Lane, Nelson, Clem, Brock, Siow, Wattenberg, Telang, Chesney: "Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases." in: The Journal of biological chemistry, Vol. 284, Issue 36, pp. 24223-32, (2009) (PubMed).

Blum, Jacob-Hirsch, Amariglio, Rechavi, Kloog: "Ras inhibition in glioblastoma down-regulates hypoxia-inducible factor-1alpha, causing glycolysis shutdown and cell death." in: Cancer research, Vol. 65, Issue 3, pp. 999-1006, (2005) (PubMed).

Target Details for PFKFB4

Target: PFKFB4 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 4 (PFKFB4))

Alternative Name: PFKFB4 (PFKFB4 Products)

Synonyms: MGC69186 antibody, MGC81068 antibody, PFKFB4 antibody, zgc:123288 antibody, C230090D14 antibody, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 antibody, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 S homeolog antibody, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4a antibody, pfkfb4 antibody, pfkfb4.S antibody, PFKFB4 antibody, pfkfb4a antibody, f264 antibody, Pfkfb4 antibody

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

Molecular Weight: 54040

Gene ID: 5210

NCBI Accession: NP_004558

UniProt: Q16877