PFKFB3 antibody (C-Term)

Catalog No. ABIN392768

Product Details anti-PFKFB3 Antibody

Target: PFKFB3 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3))

Binding Specificity: AA 454-484, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKFB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PFKFB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 454-484 amino acids from the C-terminal region of human PFKFB3.

Clone: RB04030

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-PFKFB3 antibody (ABIN392768)

Heydasch, Kessler, Warnke, Eschrich, Scholz, Bigl: "Functional diversity of PFKFB3 splice variants in glioblastomas." in: PloS one, Vol. 16, Issue 7, pp. e0241092, (2021) (PubMed).

Lee, Lee, Yun, Jang, Kang, Kim, Choi, Park: "Silver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species." in: International journal of nanomedicine, Vol. 11, pp. 55-68, (2016) (PubMed).

Reddy, Fernandes, Deshpande, Weisberg, Inguilizian, Abdel-Wahab, Kung, Levine, Griffin, Sattler: "The JAK2V617F oncogene requires expression of inducible phosphofructokinase/fructose-bisphosphatase 3 for cell growth and increased metabolic activity." in: Leukemia, Vol. 26, Issue 3, pp. 481-9, (2012) (PubMed).

Ando, Uehara, Kogure, Asano, Nakajima, Abe, Kawauchi, Tanaka: "Interleukin 6 enhances glycolysis through expression of the glycolytic enzymes hexokinase 2 and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3." in: Journal of Nippon Medical School = Nippon Ika Daigaku zasshi, Vol. 77, Issue 2, pp. 97-105, (2010) (PubMed).

Yamasaki, Hayashi, Okamoto, Osanai, Lee: "Insulin-independent promotion of chemically induced hepatocellular tumor development in genetically diabetic mice." in: Cancer science, Vol. 101, Issue 1, pp. 65-72, (2010) (PubMed).

Clem, Telang, Clem, Yalcin, Meier, Simmons, Rasku, Arumugam, Dean, Eaton, Lane, Trent, Chesney: "Small-molecule inhibition of 6-phosphofructo-2-kinase activity suppresses glycolytic flux and tumor growth." in: Molecular cancer therapeutics, Vol. 7, Issue 1, pp. 110-20, (2008) (PubMed).

Telang, Yalcin, Clem, Bucala, Lane, Eaton, Chesney: "Ras transformation requires metabolic control by 6-phosphofructo-2-kinase." in: Oncogene, Vol. 25, Issue 55, pp. 7225-34, (2006) (PubMed).

Target Details for PFKFB3

Target: PFKFB3 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3))

Alternative Name: PFKFB3 (PFKFB3 Products)

Synonyms: IPFK2 antibody, PFK2 antibody, E330010H22Rik antibody, iPFK-2 antibody, uPFK-2 antibody, PFKFB3 antibody, zgc:73257 antibody, wu:fc31e01 antibody, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 antibody, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 S homeolog antibody, PFKFB3 antibody, Pfkfb3 antibody, pfkfb3.S antibody, pfkfb3 antibody, PTRG_09894 antibody, Tsp_06710 antibody

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

Molecular Weight: 59609

Gene ID: 5209

NCBI Accession: NP_001138915, NP_004557

UniProt: Q16875

Pathways: AMPK Signaling, Regulation of Carbohydrate Metabolic Process