Hexokinase 1 antibody (N-Term)

Catalog No. ABIN392759

Product Details anti-Hexokinase 1 Antibody

Target: Hexokinase 1 (HK1)

Binding Specificity: AA 78-108, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This HK1 (Hexokinase) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-108 amino acids from the N-terminal region of human HK1 (Hexokinase).

Clone: RB3921-RB3922

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:2000. WB: 1:1000. WB: 1:1000. IHC-P-Leica: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Hexokinase 1 antibody (ABIN392759)

Lynn, Wang, Markesbery, Lovell: "Quantitative changes in the mitochondrial proteome from subjects with mild cognitive impairment, early stage, and late stage Alzheimer's disease." in: Journal of Alzheimer's disease : JAD, Vol. 19, Issue 1, pp. 325-39, (2010) (PubMed).

Target Details for Hexokinase 1

Target: Hexokinase 1 (HK1)

Alternative Name: HK1 (Hexokinase) (HK1 Products)

Background: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. The hexokinase gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of the hexokinase gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus, the remainder of the protein is identical among all the isoforms. HK1 encodes the ubiquitously expressed isoform. Its 5' end includes an exon which is unique to this transcript and which encodes a distinct N-terminus that contains the porin binding domain (PBD). The porin binding domain mediates association with the mitochondrial membrane.

Molecular Weight: 102486

Gene ID: 3098

NCBI Accession: NP_000179, NP_277031, NP_277032, NP_277033, NP_277035

UniProt: P19367

Pathways: Carbohydrate Homeostasis, Warburg Effect