NEK1 antibody (C-Term)

Catalog No. ABIN392648

Product Details anti-NEK1 Antibody

Target: NEK1 (NIMA-Related Kinase 1 (NEK1))

Binding Specificity: AA 1165-1196, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: M

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This NEK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1165-1196 amino acids from the C-terminal region of human NEK1.

Clone: RB2957

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:500. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for NEK1

Target: NEK1 (NIMA-Related Kinase 1 (NEK1))

Alternative Name: NEK1 (NEK1 Products)

Synonyms: D8Ertd790e antibody, kat antibody, NY-REN-55 antibody, SRPS2 antibody, SRPS2A antibody, ATNEK1 antibody, F20D21.32 antibody, F20D21_32 antibody, NIMA-RELATED KINASE1 antibody, NIMA-related serine/threonine kinase 1 antibody, NIMA (never in mitosis gene a)-related expressed kinase 1 antibody, NIMA related kinase 1 antibody, NIMA-related kinase 1 antibody, NIMA-related serine/threonine kinase 1 antibody, Nek1 antibody, NEK1 antibody

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Molecular Weight: 142828

Gene ID: 4750

NCBI Accession: NP_001186326, NP_001186327, NP_001186328, NP_001186329, NP_036356

UniProt: Q96PY6