UHMK1 antibody (N-Term)

Catalog No. ABIN392641

Product Details anti-UHMK1 Antibody

Target: UHMK1 (U2AF Homology Motif (UHM) Kinase 1 (UHMK1))

Binding Specificity: AA 1-30, N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UHMK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This KIST (KIS) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human KIST (KIS).

Clone: RB3367

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-UHMK1 antibody (ABIN392641)

Chen, Xie, Gu, Cai, Wang, Xie, Dong, He, Zhong, Lin, Huang: "Upregulated WDR5 promotes proliferation, self-renewal and chemoresistance in bladder cancer via mediating H3K4 trimethylation." in: Scientific reports, Vol. 5, pp. 8293, (2015) (PubMed).

Lee, Kay: "PI 3-kinase/Rac1 and ERK1/2 regulate FGF-2-mediated cell proliferation through phosphorylation of p27 at Ser10 by KIS and at Thr187 by Cdc25A/Cdk2." in: Investigative ophthalmology & visual science, Vol. 52, Issue 1, pp. 417-26, (2011) (PubMed).

Nakamura, Yamashita, Yokota, Hirano, Ono, Fujie, Shibata, Niimi, Suyama, Maddali, Asai, Yamashita, Iguchi, Ohnishi: "Development and pharmacologic characterization of deoxybromophospha sugar derivatives with antileukemic activity." in: Investigational new drugs, Vol. 28, Issue 4, pp. 381-91, (2010) (PubMed).

Takemura, Nakamura, Yokota, Hirano, Ono, Shigeno, Fujisawa, Ohnishi: "Reduction of Raf kinase inhibitor protein expression by Bcr-Abl contributes to chronic myelogenous leukemia proliferation." in: The Journal of biological chemistry, Vol. 285, Issue 9, pp. 6585-94, (2010) (PubMed).

Nakamura, Hirano, Okinaka, Takemura, Yokota, Ono, Shigeno, Shibata, Fujisawa, Ohnishi: "The FOXM1 transcriptional factor promotes the proliferation of leukemia cells through modulation of cell cycle progression in acute myeloid leukemia." in: Carcinogenesis, Vol. 31, Issue 11, pp. 2012-21, (2010) (PubMed).

Petrovic, Costa, Lau, Raychaudhuri, Tyner: "FoxM1 regulates growth factor-induced expression of kinase-interacting stathmin (KIS) to promote cell cycle progression." in: The Journal of biological chemistry, Vol. 283, Issue 1, pp. 453-60, (2007) (PubMed).

Target Details for UHMK1

Target: UHMK1 (U2AF Homology Motif (UHM) Kinase 1 (UHMK1))

Alternative Name: KIST (KIS) (UHMK1 Products)

Synonyms: UHMK1 antibody, zgc:153241 antibody, KIS antibody, KIST antibody, P-CIP2 antibody, 4732477C12Rik antibody, 4930500M09Rik antibody, AA673513 antibody, AI449218 antibody, AU021979 antibody, Kist antibody, U2AF homology motif kinase 1 antibody, U2AF homology motif (UHM) kinase 1 antibody, UHMK1 antibody, uhmk1 antibody, Uhmk1 antibody

Background: Upon serum stimulation, KIS, a member of the Ser/Thr protein kinase family, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. This protein, which contains 1 RRM (RNA recognition motif)domain, may be involved in trafficking and/or processing of RNA. KIS is widely expressed, with highest levels in skeletal muscle, kidney, placenta and peripheral blood leukocytes.

Molecular Weight: 46546

Gene ID: 127933

NCBI Accession: NP_001171692, NP_653225, NP_787062

UniProt: Q8TAS1