Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This PIP5K1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PIP5K1B.
PIP5K1B
Reactivity: Human, Mouse, Rat
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date
6 months
Qin, Li, Pan, Wu: "Regulation of phosphatidylinositol kinases and metabolism by Wnt3a and Dvl." in: The Journal of biological chemistry, Vol. 284, Issue 34, pp. 22544-8, (2009) (PubMed).
Bolomini-Vittori, Montresor, Giagulli, Staunton, Rossi, Martinello, Constantin, Laudanna: "Regulation of conformer-specific activation of the integrin LFA-1 by a chemokine-triggered Rho signaling module." in: Nature immunology, Vol. 10, Issue 2, pp. 185-94, (2009) (PubMed).
Target
PIP5K1B
(Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, beta (PIP5K1B))
MSS4 antibody, STM7 antibody, AI844522 antibody, Pip5k1a antibody, Pipk5a antibody, Pipk5b antibody, MGC80809 antibody, pip5k1b antibody, zgc:101046 antibody, wu:fj29b05 antibody, wu:fj93b05 antibody, zgc:92316 antibody, phosphatidylinositol-4-phosphate 5-kinase type 1 beta antibody, phosphatidylinositol-4-phosphate 5-kinase, type 1 beta antibody, phosphatidylinositol-4-phosphate 5-kinase, type I, beta L homeolog antibody, phosphatidylinositol-4-phosphate 5-kinase, type I, beta a antibody, phosphatidylinositol-4-phosphate 5-kinase, type I, beta antibody, phosphatidylinositol 4-phosphate 5-kinase type-1 beta antibody, phosphatidylinositol-4-phosphate 5-kinase, type I, beta b antibody, PIP5K1B antibody, Pip5k1b antibody, pip5k1b.L antibody, pip5k1ba antibody, pip5k1b antibody, LOC100472550 antibody, pip5k1bb antibody
Background
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.