PIK3C3 antibody (N-Term)

Catalog No. ABIN392555

Product Details anti-PIK3C3 Antibody

Target: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Binding Specificity: AA 24-53, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3C3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: M, Pig, Rat, X

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human PI3KC3.

Clone: RB1695

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PIK3C3 antibody (ABIN392555)

Wang, Li, Du, Xu, Wang, Zhang, Xu, Zeng, Mao, Cao: "The Class I PI3K inhibitor S14161 induces autophagy in malignant blood cells by modulating the Beclin 1/Vps34 complex." in: Journal of pharmacological sciences, Vol. 134, Issue 4, pp. 197-202, (2018) (PubMed).

Won, Yen, Lin, Jiang-Shieh, Lin, Chen, Su: "Autophagy mediates cytotoxicity of human colorectal cancer cells treated with garcinielliptone FC." in: Journal of cellular physiology, Vol. 233, Issue 1, pp. 497-505, (2017) (PubMed).

Lin, Wang, Hsu, Cho, Yang, Chien: "Capsaicin Induces Autophagy and Apoptosis in Human Nasopharyngeal Carcinoma Cells by Downregulating the PI3K/AKT/mTOR Pathway." in: International journal of molecular sciences, Vol. 18, Issue 7, (2017) (PubMed).

Zhang, Morgan, Chen, Choksi, Liu: "Induction of autophagy is essential for monocyte-macrophage differentiation." in: Blood, Vol. 119, Issue 12, pp. 2895-905, (2012) (PubMed).

McLeod, Zhou, Li, Wang, He: "The class III kinase Vps34 promotes T lymphocyte survival through regulating IL-7R? surface expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 10, pp. 5051-61, (2011) (PubMed).

Zhou, Wang, Hasegawa, Amin, Han, Kaneko, He, Wang: "Deletion of PIK3C3/Vps34 in sensory neurons causes rapid neurodegeneration by disrupting the endosomal but not the autophagic pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 20, pp. 9424-9, (2010) (PubMed).

Wang, Whiteman, Lian, Wang, Singh, Huang, Denmark: "A non-canonical MEK/ERK signaling pathway regulates autophagy via regulating Beclin 1." in: The Journal of biological chemistry, Vol. 284, Issue 32, pp. 21412-24, (2009) (PubMed).

Miselli, Negri, Gronchi, Losa, Conca, Brich, Fumagalli, Fiore, Casali, Pierotti, Tamborini, Pilotti: "Is autophagy rather than apoptosis the regression driver in imatinib-treated gastrointestinal stromal tumors?" in: Translational oncology, Vol. 1, Issue 4, pp. 177-86, (2008) (PubMed).

Liang, Feng, Ku, Dotan, Canaani, Oh, Jung: "Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG." in: Nature cell biology, Vol. 8, Issue 7, pp. 688-99, (2006) (PubMed).

Target Details for PIK3C3

Target: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Alternative Name: PI3KC3 (PIK3C3 Products)

Synonyms: im:7147158 antibody, wu:fc13g06 antibody, wu:fz51e12 antibody, zgc:112016 antibody, zgc:113009 antibody, VPS34 antibody, hVps34 antibody, 5330434F23Rik antibody, Vps34 antibody, phosphatidylinositol 3-kinase catalytic subunit type 3 antibody, phosphatidylinositol 3-kinase catalytic subunit type 3 L homeolog antibody, phosphatidylinositol 3-kinase, catalytic subunit type 3 antibody, PIK3C3 antibody, pik3c3.L antibody, pik3c3 antibody, Pik3c3 antibody

Background: PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.

Molecular Weight: 101549

Gene ID: 5289

NCBI Accession: NP_002638

UniProt: Q8NEB9

Pathways: AMPK Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Toll-Like Receptors Cascades, Autophagy